Thesis Title: Impact of diet, age and health status on circulating amino acids.

Elevated Branched-chain amino acids (BCAA) has been shown to correlate with an increased risk of insulin resistance (IR) and T2DM. Metabolic profiling demonstrates that BCAA have emerged as potential biomarker of a metabolic dysregulated status rather than an initiating event in the causal chain, as their concentrations appear to be elevated many years prior to the onset of T2DM. However, whether circulatory concentrations of AA are impacted by quality and quantity of dietary protein, protein digestion rate, disease states and ageing related phenomenon remains an area of controversy. Thus in the synchronization of the exploration, this thesis is hypothesized to unveil the novel interplay between manipulation of dietary protein intake and related twists in protein digestion to their reflective circulatory AA and metabolic health. Primarily, decisive acute and chronic response of circulatory AA due to diverse dietary modification will be investigated. Subsequently, the changes in circulatory AA including BCAA will be investigated during pre-disease states as a means of dietary weight loss of overweight adults. As an outcome if dietary alterations or improvement in metabolic health influence fasting BCAA levels, then this tactic could be realistic to apply in future studies to explore the mechanisms associating fasting levels of BCAA to altered metabolic state or as unique methods for improving insulin sensitivity. Apart from the impact of protein qualities and quantities, how digestion related multi-phenomena affect absorption of its constituent AA into the circulation will be investigated exclusively. Profiling of BCAA along with other AA in ageing and subjects with digestive intolerance is opted to clarify the grounds of apparent improvement in metabolic health.


Liggins Institute, University of Auckland and Department of Food Technology and Nutritional Science, Mawlana Bhashani Science and Technology University (Bangladesh)