Thesis Title: Impact of dietary proteins on digestive tolerance and novel measures of gastrointestinal and systemic inflammation.

Dietary components may play an important role to trigger inflammation, for example digestion of some protein like casein (milk derived) and gliadin (wheat derived) produce peptides with opioid activity which may predispose susceptible individuals to inflammation and systemic oxidation. β -casein protein from milk has basically 2 variants –A1 and A2 casein which differs by one amino acid -proline67 (A2 β-casein) to histidine67 (A1 β-casein) so it is digested differently. The digestion of A1 variant but not A2 variant of bovine β-casein releases β–casmorphin-7 (BCM-7) which has opioid or morphine like activity that can bind opioid receptors expressed in digestive, immune and neural tissue. Thus my study aims to understand the impacts of dietary protein on digestive tolerance and systemic inflammation focussing on A1 verses A2 β- casein protein. In addition, the study aims to use metabolomics approach to ascertain new biomarkers of dairy intolerance and understand the physiological and metabolic changes in lactose and protein intolerance with both acute and long term ingestion of the dairy.


University of Auckland